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anti cd209 apc  (Miltenyi Biotec)


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    Miltenyi Biotec anti cd209 apc
    Anti Cd209 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd209+apc/CD209+(DC-SIGN)+Antibody%2C+anti-human%2C+REAfinity/pmc12339949-396-9-13
    Average 93 stars, based on 17 article reviews
    anti cd209 apc - by Bioz Stars, 2026-09
    93/100 stars

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    Cell Analysis:

    Article Title: Melanoma Affects the Composition of Blood Cell-Derived Extracellular Vesicles
    Article Snippet: For analysis, the flow cytometer MACSQuant Analyzer 10 with the corresponding software (Miltenyi Biotec) was used. .. For cell analysis, 5 × 105 monocytes and 5 × 105 moDCs were each stained with CD14-FITC (Miltenyi Biotec Cat# 130- 080-701 Lot# RRID:AB_244303), CD40-APC (Miltenyi Biotec Cat# 130-094-137 Lot# RRID:AB_10828346), and CD86-PE (Miltenyi Biotec Cat# 130-094-877 Lot# RRID:AB_10839702) or with CD209-APC (Miltenyi Biotec Cat# 130-092-871 Lot# RRID:AB_871640). .. Stainings were performed in a total volume of 110 μl with final antibody dilutions of 1:11 with FcR Blocking reagent (Miltenyi Biotec) in PEB (PBS with 0.5% BSA and 2 mM EDTA) for 10 min at 4°C.

    Article Title: Melanoma Affects the Composition of Blood Cell-Derived Extracellular Vesicles
    Article Snippet: For analysis, the flow cytometer MACSQuant Analyzer 10 with the corresponding software (Miltenyi Biotec) was used. .. For cell analysis, 5 × 10 5 monocytes and 5 × 10 5 moDCs were each stained with CD14-FITC (Miltenyi Biotec Cat# 130-080-701 Lot# RRID:AB_244303), CD40-APC (Miltenyi Biotec Cat# 130-094-137 Lot# RRID:AB_10828346), and CD86-PE (Miltenyi Biotec Cat# 130-094-877 Lot# RRID:AB_10839702) or with CD209-APC (Miltenyi Biotec Cat# 130-092-871 Lot# RRID:AB_871640). .. Stainings were performed in a total volume of 110 μl with final antibody dilutions of 1:11 with FcR Blocking reagent (Miltenyi Biotec) in PEB (PBS with 0.5% BSA and 2 mM EDTA) for 10 min at 4°C.

    Staining:

    Article Title: Melanoma Affects the Composition of Blood Cell-Derived Extracellular Vesicles
    Article Snippet: For analysis, the flow cytometer MACSQuant Analyzer 10 with the corresponding software (Miltenyi Biotec) was used. .. For cell analysis, 5 × 105 monocytes and 5 × 105 moDCs were each stained with CD14-FITC (Miltenyi Biotec Cat# 130- 080-701 Lot# RRID:AB_244303), CD40-APC (Miltenyi Biotec Cat# 130-094-137 Lot# RRID:AB_10828346), and CD86-PE (Miltenyi Biotec Cat# 130-094-877 Lot# RRID:AB_10839702) or with CD209-APC (Miltenyi Biotec Cat# 130-092-871 Lot# RRID:AB_871640). .. Stainings were performed in a total volume of 110 μl with final antibody dilutions of 1:11 with FcR Blocking reagent (Miltenyi Biotec) in PEB (PBS with 0.5% BSA and 2 mM EDTA) for 10 min at 4°C.

    Article Title: Melanoma Affects the Composition of Blood Cell-Derived Extracellular Vesicles
    Article Snippet: For analysis, the flow cytometer MACSQuant Analyzer 10 with the corresponding software (Miltenyi Biotec) was used. .. For cell analysis, 5 × 10 5 monocytes and 5 × 10 5 moDCs were each stained with CD14-FITC (Miltenyi Biotec Cat# 130-080-701 Lot# RRID:AB_244303), CD40-APC (Miltenyi Biotec Cat# 130-094-137 Lot# RRID:AB_10828346), and CD86-PE (Miltenyi Biotec Cat# 130-094-877 Lot# RRID:AB_10839702) or with CD209-APC (Miltenyi Biotec Cat# 130-092-871 Lot# RRID:AB_871640). .. Stainings were performed in a total volume of 110 μl with final antibody dilutions of 1:11 with FcR Blocking reagent (Miltenyi Biotec) in PEB (PBS with 0.5% BSA and 2 mM EDTA) for 10 min at 4°C.



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    (A) The CD163 + <t>CD209</t> + immunophenotype of MDM-M2 macrophages was characterized and compared to that of their monocyte precursors (scatter plot). The PD-L1 expression on MDM-M2 macrophages was confirmed by immunostaining and analyzed using flow cytometry, with CD14 + monocytes serving as a negative control (histogram plots). (B) MC9999 CAR T-cells exhibited cytotoxicity against MDM-M2 macrophages but not CD14 + monocytes, as determined via a CD107a degranulation assay. (C) The release of granzyme B was significantly increased when MC9999 CAR T-cells were cultured with MDM-M2 macrophages. The data, plotted as mean ± SEM of triplicate sampling, are representative of three independent experiments and analyzed using the multiple T test (***, p < 0.001; ns, no significance). (D) The impedance-based killing assay demonstrated the direct killing of MDM-M2 macrophages by MC9999 CAR T-cells, as evidenced by a significant decrease in the cell index upon the addition of the CAR T-cells to cultured MDM-M2 macrophages. The data are representative of three independent experiments. (E) TAMs isolated from a surgically resected GBM tumor displayed the expected CD163 + CD209 + immunophenotype upon immunostaining. (F) The CD163 + CD209 + gated TAMs were highly positive for PD-L1 at the cell surface. CD14 + monocytes served as the negative control for both immunophenotypic characterization and PD-L1 staining. (G) Evaluation via the CD107a degranulation assay revealed the MC9999 CAR T-cells, derived from healthy donor T cells, elicited cytotoxicity against the TAMs extracted from GBM tumor.
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    (A) The CD163 + <t>CD209</t> + immunophenotype of MDM-M2 macrophages was characterized and compared to that of their monocyte precursors (scatter plot). The PD-L1 expression on MDM-M2 macrophages was confirmed by immunostaining and analyzed using flow cytometry, with CD14 + monocytes serving as a negative control (histogram plots). (B) MC9999 CAR T-cells exhibited cytotoxicity against MDM-M2 macrophages but not CD14 + monocytes, as determined via a CD107a degranulation assay. (C) The release of granzyme B was significantly increased when MC9999 CAR T-cells were cultured with MDM-M2 macrophages. The data, plotted as mean ± SEM of triplicate sampling, are representative of three independent experiments and analyzed using the multiple T test (***, p < 0.001; ns, no significance). (D) The impedance-based killing assay demonstrated the direct killing of MDM-M2 macrophages by MC9999 CAR T-cells, as evidenced by a significant decrease in the cell index upon the addition of the CAR T-cells to cultured MDM-M2 macrophages. The data are representative of three independent experiments. (E) TAMs isolated from a surgically resected GBM tumor displayed the expected CD163 + CD209 + immunophenotype upon immunostaining. (F) The CD163 + CD209 + gated TAMs were highly positive for PD-L1 at the cell surface. CD14 + monocytes served as the negative control for both immunophenotypic characterization and PD-L1 staining. (G) Evaluation via the CD107a degranulation assay revealed the MC9999 CAR T-cells, derived from healthy donor T cells, elicited cytotoxicity against the TAMs extracted from GBM tumor.
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    (A) The CD163 + CD209 + immunophenotype of MDM-M2 macrophages was characterized and compared to that of their monocyte precursors (scatter plot). The PD-L1 expression on MDM-M2 macrophages was confirmed by immunostaining and analyzed using flow cytometry, with CD14 + monocytes serving as a negative control (histogram plots). (B) MC9999 CAR T-cells exhibited cytotoxicity against MDM-M2 macrophages but not CD14 + monocytes, as determined via a CD107a degranulation assay. (C) The release of granzyme B was significantly increased when MC9999 CAR T-cells were cultured with MDM-M2 macrophages. The data, plotted as mean ± SEM of triplicate sampling, are representative of three independent experiments and analyzed using the multiple T test (***, p < 0.001; ns, no significance). (D) The impedance-based killing assay demonstrated the direct killing of MDM-M2 macrophages by MC9999 CAR T-cells, as evidenced by a significant decrease in the cell index upon the addition of the CAR T-cells to cultured MDM-M2 macrophages. The data are representative of three independent experiments. (E) TAMs isolated from a surgically resected GBM tumor displayed the expected CD163 + CD209 + immunophenotype upon immunostaining. (F) The CD163 + CD209 + gated TAMs were highly positive for PD-L1 at the cell surface. CD14 + monocytes served as the negative control for both immunophenotypic characterization and PD-L1 staining. (G) Evaluation via the CD107a degranulation assay revealed the MC9999 CAR T-cells, derived from healthy donor T cells, elicited cytotoxicity against the TAMs extracted from GBM tumor.

    Journal: bioRxiv

    Article Title: Advancing CAR T-Cell Therapy: Simultaneously Attack Tumor and Immunosuppressive Cells in the Tumor Microenvironment

    doi: 10.1101/2024.03.25.586653

    Figure Lengend Snippet: (A) The CD163 + CD209 + immunophenotype of MDM-M2 macrophages was characterized and compared to that of their monocyte precursors (scatter plot). The PD-L1 expression on MDM-M2 macrophages was confirmed by immunostaining and analyzed using flow cytometry, with CD14 + monocytes serving as a negative control (histogram plots). (B) MC9999 CAR T-cells exhibited cytotoxicity against MDM-M2 macrophages but not CD14 + monocytes, as determined via a CD107a degranulation assay. (C) The release of granzyme B was significantly increased when MC9999 CAR T-cells were cultured with MDM-M2 macrophages. The data, plotted as mean ± SEM of triplicate sampling, are representative of three independent experiments and analyzed using the multiple T test (***, p < 0.001; ns, no significance). (D) The impedance-based killing assay demonstrated the direct killing of MDM-M2 macrophages by MC9999 CAR T-cells, as evidenced by a significant decrease in the cell index upon the addition of the CAR T-cells to cultured MDM-M2 macrophages. The data are representative of three independent experiments. (E) TAMs isolated from a surgically resected GBM tumor displayed the expected CD163 + CD209 + immunophenotype upon immunostaining. (F) The CD163 + CD209 + gated TAMs were highly positive for PD-L1 at the cell surface. CD14 + monocytes served as the negative control for both immunophenotypic characterization and PD-L1 staining. (G) Evaluation via the CD107a degranulation assay revealed the MC9999 CAR T-cells, derived from healthy donor T cells, elicited cytotoxicity against the TAMs extracted from GBM tumor.

    Article Snippet: The analysis included staining for BUV395-CD14 (clone M5E2, BD Horizon, USA), APC-CD209 (clone DCN46, BD Pharmingen, USA), AF488-CD163 (clone MAC2-158, BD Pharmingen, USA), and BV650-PDL-1 (clone29E.2A3, Biolegend, USA).

    Techniques: Expressing, Immunostaining, Flow Cytometry, Negative Control, Degranulation Assay, Cell Culture, Sampling, Isolation, Staining, Derivative Assay